Figure 7. The +L264 and S252F mutations did not affect surface mAb 299 and mAb 270 antibody binding.

A-D, [¹²⁵I]secondary antibody labelling of the S252F, +L264 and WT receptors. Each mutant was paired with a matched WT control. We used both anti-α4 (mAb 299) and anti-β2 (mAb 270) primary antibodies to measure surface receptor expression. Filled bars show ¹²⁵I labelling of the receptors treated with both the primary (1°) and secondary (2°) antibodies. Open bars show ¹²⁵I labelling of the receptors treated with the secondary (2°) antibody only. Error bars are +s.e.m.