Table 4.

Uptake of butyrate and l-lactate in oocytes injected with rat MCT1 cRNA

Conditions		Uptake (pmol per oocyte (2.5 min)−1)
	Inhibitors	n	Butyrate	l-Lactate
Water-injected (control)	none	6	82 ± 11	4 ± 1
MCT1 cRNA injected	none	6	195 ± 31*	32 ± 11*
	5 mm butyrate	6	—	4.6 ± 0.2
	5 mml-lactate	6	43 ± 8	—
	5 mm pyruvate	6	40 ± 6	2.1 ± 0.4

Oocytes were injected with either 40 nl of sterile water or 40 nl (20 ng) rat MCT1 cRNA (Broöer et al. 1997) and incubated for 4 days. The uptake of 0.1 mml-[U-¹⁴C]lactate or 1 mm[U-¹⁴C]butyrate was then measured in modified Barth's solution at pH 7.0. Rates of uptake were also determined in the presence or absence of the indicated inhibitor (5 mm). After 2.5 min incubation the reaction was stopped by washing the oocytes 5 times with 1 ml ice cold stop solution (modified Barth's solution pH 7.0 with either 0.1 mml-lactate or 1 mm butyrate). n, number of oocytes

^*Statistically significant (P < 0.05) from control (water-injected) oocytes.