Table 4.

Designs for expression profiling of Y. pestis

Environmental perturbation	Description	Reference (s)
Stimulon analysis
Temperature shift		[8, 9]
Vegetative growth temperatures	Shift from 26°C to 37°C for 3 hr
Heat shock	Shift from 37°C to 45°C for 10 min
Cold shock	Shift from 37°C to 10°C for 1 hr
Osmotic stress		[10]
High osmolarity	Treatment with 0.5 M sorbitol for 20 min
High salinity	Treatment with 0.5 M NaCl for 20 min
Oxidative stress	Treatment with 1 mM H2O2 for 30 min at 26°C
Mild acid stress	Shift from pH7.2 to pH 5.5 for 10 min
Low Mg2+	Growth under 10 μM Mg2+	[15]
Iron starvation	Treatment with 100 μM of 2, 2'-dipyridyl for 30 min	[11]
26°C	26°C cell cultures
37°C	37°C cell cultures
Minimal versus rich medium	TMH versus BHI medium
Exponential phase	Growth to an A620 nm of approximately 0.8
Stationary phase	Growth to an A620 nm of approximately 2.0
Growth phase	Exponential versus stationary phase
Minimum medium	Growth in TMH medium
Rich medium	Growth in BHI medium
Antibiotics treatment	10× MIC concentration of antibiotics for 30 min
Streptomycin	37°C cell cultures	[12]
chloramphenicol	37°C cell cultures
Tetracycline	37°C cell cultures
Antibacterial peptide	26°C cell cultures
Regulon analysis
PhoP regulon	phoP mutant versus Wide-type (WT) strain
pH5.5	Growth at pH5.5
Low Mg2+	Growth under 10 μM Mg2+	[15]
Fur regulon	fur mutant versus WT strain	[11]
26°C	26°C cell cultures with addition of 40 μM of FeCl3 for 30 min
37°C	37°C cell cultures with addition of 40 μM of FeCl3 for 30 min
OxyR regulon	oxyR mutant versus WT strain upon exposure to 1 mM H2O2 for 30 min at 26°C
OmpR regulon	ompR mutant versus WT strain
High osmolarity	Upon exposure to 0.5 M sorbitol for 20 min
High salinity	Upon exposure to 0.5 M NaCl for 20 min