Table 7.
Real-time PCR validation of microarray negative results
| Gene* (Clone No. on Microarray) | Hog 1/hog 2 | Sow 1/sow 2 | Hog 3/hog 4 | Sow 3/sow 4 | ||||
| Change (test/control) | P value | Change (test/control) | P value | Change (test/control) | P value | Change (test/control) | P value | |
| SCD (rpfat_16685) | 1.56 | 0.1680 | 0.77 | 0.2119 | 1.49 | 0.0822 | 1.23 | 0.9943 |
| HSL (rpfat_11096) | 1.29 | 0.0679 | 1.11 | 0.2989 | 1.29 | 0.3462 | 1.06 | 0.5294 |
| PMP22a (rpfat_18575) | 1.15 | 0.6198 | 1.16 | 0.3178 | 1.24 | 0.6900 | 1.46 | 0.1175 |
| PHPT1b (rpfat_15312) | 0.75 | 0.1218 | 0.95 | 0.5811 | 1.24 | 0.4694 | 1.46 | 0.4869 |
* The full names of each gene are listed in Tables 2-5.
a : PMP22: peripheral myelin protein 22
b :PHPT1: Phosphohistidine phosphatase 1
Three genes (HSL, PMP22 and PHPT1) were confirmed by real-time PCR.