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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Sep 26;1780(1):41–50. doi: 10.1016/j.bbagen.2007.09.011

Figure 4.

Figure 4

Normalized OH stretch spectra (left) at 303 K (heavy lines) and 273 K (thin lines) for 90% D2O/10% H2O (v/v) (black), 80% D2O/20% H2O (v/v) (gray), 70% D2O/30% H2O (v/v) (light gray). Free energy analysis of the transition from high-energy to low-energy H-bonding as a function of temperature for the same conditions is shown on the right. Measurements were performed in duplicate for all conditions. Error bars represent one standard deviation.