Figure 1.

Identification of a nonsymptomatic CChMVd RNA. (A) Differential response of two chrysanthemum cultivars after inoculation with a nucleic acid preparation of the severe CChMVd-S strain. (B) Analysis of chrysanthemum RNAs by denaturing PAGE and ethidium bromide staining (Left) or Northern-blot hybridization with a probe complementary to CChMVd-S RNA (Right). Lane 1, DNA marker of 392 nt; lanes 2 and 3, RNAs from healthy and symptomatic “Bonnie Jean” plants, respectively; lanes 4 and 5, RNAs from symptomless “Yellow Delaware” plants uninoculated and inoculated with a CChMVd-S RNA preparation, respectively. The migration of the circular (c) and linear (l) viroid forms is indicated at the right.