Figure 4.

Infectivity of some representative CChMVd-NS variants and self-cleavage of their monomeric plus (+) and minus (−) RNAs. Dot-blot hybridization signals correspond to undiluted extracts and 1:10 dilutions (left and right spot columns, respectively). The extent of self-cleavage is expressed as the fraction (%) of the primary transcript that self-cleaved during in vitro transcription. The nonsymptomatic CM 20–1 variant was obtained from the symptomatic CM 20 variant by introducing the change UUUC82–85 → GAAA.