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. 2000 Dec 1;116(6):769–780. doi: 10.1085/jgp.116.6.769

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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A glutamate at position 204 of the Molf or Rolf channel produced voltage-dependent gating similar to that of the Brod channel. To analyze the data from multiple patches, the dose–response curve from each patch was fit with the Hill relation, and each set of data was normalized to the cGMP K_1/2 at +100 mV for that patch. Each different symbol represents the normalized data points from a single patch; open symbols for −100 mV and closed symbols for +100 mV. All points for each voltage were re-fit with the Hill equation, setting the normalized K_1/2 for +100 mV at 1.0; a change in the normalized K_1/2 at −100 mV indicates voltage-dependent gating. (A) Brod channel (six patches): at +100 mV, n = 2.0; and at −100 mV, the normalized cGMP K_1/2 = 1.4 and n = 2.0. (B) Rolf channel (six patches): at +100 mV, n = 2.8; and at −100 mV, the normalized cGMP K_1/2 = 1.0 and n = 2.8. (C) Rolf G204E channel (2 patches): at +100 mV, n = 1.7; and at −100 mV, the normalized cGMP K_1/2 = 1.7 and n = 1.7.