Figure 4.

Amplified products of in vivo cis- and trans-splicing. RT-PCR was used to amplify the RNA products of splicing from yeast cells expressing the indicated ribozyme and target genes. Bands migrating at a rate expected for DNA fragments 632 nt in length (indicated by an arrow) correspond to spliced products, and the major slower migrating bands in lanes 2 and 3 correspond to unspliced cis-acting ribozyme and inactive ΔP5abc derivative, respectively. A faint band corresponding to 632 nt was seen in lane 4.