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. Author manuscript; available in PMC: 2008 Feb 6.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Jan 30;341(4):904–910. doi: 10.1016/j.bbrc.2006.01.093

Fig. 1.

Fig. 1

Geranylgeranylated-γ5 peptide (γ5pep-gg) does not affect NMS binding to M2. [3H]NMS binding to G protein depleted CHO cell membranes expressing M2 (50 pM) was assayed in the presence or absence of γ5pep-gg (100 nM) for 60 min at room temperature as described under Materials and methods. [3H]NMS binding was measured in a standard filter binding assay. Representative result is shown from two independent experiments in duplicate. Non-specific binding was measured with 1000-fold excess atropine over NMS and was less than 5% of total counts. Scatchard plot was created with the data and the slope (1/Kd) was calculated by linear regression using the Prism program (GraphPad Software).