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. Author manuscript; available in PMC: 2008 Feb 6.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Jan 30;341(4):904–910. doi: 10.1016/j.bbrc.2006.01.093

Fig. 4.

Fig. 4

Effect of γ5 peptides on antagonist binding affinity of M2 receptor. (A) Effect of γ5pep-gg on M2 receptor binding affinity for QNB. The antagonist binding assay was performed as described in Fig. 3B legend and Materials and methods. The concentration of peptide used was 3 μM. Control represents experiments performed with the vehicle (sodium cholate). Data are expressed as means ± SEM (n = 5). Asterisks denote that NMS binding was significantly more in the presence of γ5pep-gg at these concentrations of QNB compared to either control or receptor + γ5pep-gg-scr (p < 0.05). (B) Effect of γ5pep-gg on M2 receptor binding affinity for atropine. Assay conditions were similar to those in (A). The concentration of peptide used was 3 μM. Control represents experiments performed with the vehicle (sodium cholate). Data points are expressed as means ± SEM (n = 5).