Figure 3.

The G-clamp-modified p27^kip1 S-ON demonstrates potent, dose-dependent antisense inhibition. Immunodetection of p27^kip1 and PI3K (p85 regulatory subunit) after transfection of CV-1 cells with GS3815 cytofectin (lane 1); C5-propynyl with 11 substitutions (ON8; lanes 2–4)(see Table 2 for sequences); 5MeC, 5-methylcytosine control (ON9; lanes 5–7); phenoxazine-modified (ON10; lanes 8–10), and G-clamp modified (ON4; lanes 11–13) using 2.5 μg/ml GS3815 cytofectin and a range of S-ON concentrations (30, 10, and 3 nM).