Figure 3.

Transcriptional repression by the N-terminal domain of SMRTe. (A) Schematic of the Gal4 DBD fusion constructs used in this experiment. The domains are as described in Fig. 2B. The numbers indicate amino acid residues. The seven different SMRTe N-terminal fragments (A to G) are fused into Gal4 DBD and their effects on reporter gene expression are shown in B. (B) Transcriptional repression by Gal4 DBD-SMRTe fusions. The fold repression of each construct was determined by average relative luciferase activity using Gal4 DBD as a standard in a triplicate experiment.