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. 1999 Mar 30;96(7):3525–3530. doi: 10.1073/pnas.96.7.3525

Figure 3.

Figure 3

Multiple sequence alignment of various E. coli proteins that interact with IIAGlc. Accession numbers for the various proteins are as follows: LacY, p02920; RafB, p16522; MelB, K01991; GK, M18393; and HPr, J02796. The corrected sequence of MalK is from ref. 28. Residues proposed to be essential for binding to IIAGlc are shown in blue shaded boxes. The IIAGlc contacts with GK are from ref. 12. (A) Region I alignment. The site of polyhistidine insertion (labeled insertion site) in loop IV/V of lac permease is from ref. 14. (B) Region II alignment. The site of polyhistidine insertion (labeled insertion site) in loop VI/VII is from refs. 14 and 16. Mutants in LacY resulting in loss of inducer exclusion (shown as yellow shaded circles) are from ref. 11. Mutations in MalK resulting in loss of inducer exclusion (yellow shaded circles) are from refs. 8 and 24. The old consensus is from ref. 9.