Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Dec;64(4):1195–1202. doi: 10.1073/pnas.64.4.1195

PROPERTIES OF A TEMPERATURE-SENSITIVE, RADIATION-SENSITIVE MUTANT OF Escherichia coli, II. DNA REPLICATION*

Crellin Pauling 1, Lyla Hamm 1
PMCID: PMC223268  PMID: 4916921

Abstract

We have described a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. In vitro assays have demonstrated that DNA ligase activity is markedly reduced in this strain relative to that of the parent strain. Okazaki fragments of newly replicated DNA accumulate in this mutant strain upon growth at the restrictive temperature. These results imply that the same enzyme, DNA ligase, functions both in normal semiconservative replication and in nonconservative repair replication of DNA.

Full text

PDF
1195

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gellert M. Formation of covalent circles of lambda DNA by E. coli extracts. Proc Natl Acad Sci U S A. 1967 Jan;57(1):148–155. doi: 10.1073/pnas.57.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HANAWALT P. C. Involvement of synthesis of RNA in thymineless death. Nature. 1963 Apr 20;198:286–286. doi: 10.1038/198286a0. [DOI] [PubMed] [Google Scholar]
  3. HANAWALT P. C., RAY D. S. ISOLATION OF THE GROWING POINT IN THE BACTERIAL CHROMOSOME. Proc Natl Acad Sci U S A. 1964 Jul;52:125–132. doi: 10.1073/pnas.52.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Newman J., Hanawalt P. Intermediates in T4 DNA replication in a T4 ligase deficient strain. Cold Spring Harb Symp Quant Biol. 1968;33:145–150. doi: 10.1101/sqb.1968.033.01.018. [DOI] [PubMed] [Google Scholar]
  5. Okazaki R., Okazaki T., Sakabe K., Sugimoto K., Sugino A. Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains. Proc Natl Acad Sci U S A. 1968 Feb;59(2):598–605. doi: 10.1073/pnas.59.2.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Olivera B. M., Lehman I. R. Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli. Proc Natl Acad Sci U S A. 1967 May;57(5):1426–1433. doi: 10.1073/pnas.57.5.1426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PETTIJOHN D., HANAWALT P. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. J Mol Biol. 1964 Aug;9:395–410. doi: 10.1016/s0022-2836(64)80216-3. [DOI] [PubMed] [Google Scholar]
  8. Pauling C., Hamm L. Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1495–1502. doi: 10.1073/pnas.60.4.1495. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES