Figure 5.
INCX amplifies odorant responses in squid ORNs. (A) Three successive responses to 5 mM glutamate, 5 mM glutamate in 50 μM DCB, and 5 mM glutamate, respectively, are shown for a nystatin-perforated patched squid ORN. This cell was exposed to DCB for 36 s before application of glutamate + DCB. When DCB is removed, the size of the glutamate-induced current returns to control levels. (B) The magnitude of the peak current was normalized to the peak of the control responses and plotted as a bar graph. DCB was either coapplied with glutamate (n = 8) or pre-incubated for 58 ± 21 s (n = 10). The combined DCB data show that the response to 5 mM glutamate is reduced by 26 ± 10% (n = 18; vertical stripes). In the absence of external Na+ (Li+ + Glu, diagonal stripes), 5 mM glutamate responses were reduced by 31 ± 7%. Error bars indicate SD. *P < 0.05. Solutions: ASW ext/60 TEA Na gluconate int.