Table 2.
Composition of Internal Pipette Solutions
| Internal solution | Na+ | K+ | Cl− | F− | Gluc− | TMA+ | TEA+ |
|---|---|---|---|---|---|---|---|
| Na Gluc with MgATP | 328 | 25 | 61 | 25 | 326 | 0 | 52 |
| 60 TEA Na Gluc | 355 | 0 | 61 | 25 | 330 | 0 | 60 |
| Li Gluc | 0 | 25 | 61 | 25 | 330 | 0 | 60 |
| TMA Gluc | 25 | 0 | 61 | 25 | 270 | 270 | 60 |
| TMA Cl | 25 | 0 | 365 | 25 | 0 | 365 | 0 |
All solutions were set to pH 7.2 with NaOH or TMAOH and were set to an osmolality of 780 mOsm kg−1 with d-glucose. Pipette solutions also contained 0.5 mM Ca2+, 10 mM HEPES, and 1.5 mM EGTA. Nystatin was added to various internal solutions, as described in materials and methods. Gluc, gluconate.