Table 1.
Semiquantitative assessment of XWnt8-AP binding to different Frizzled and CRD-myc-GPI proteins based on X-phos/NBT staining of live, transiently transfected COS cells
| Frizzled | Full-length protein | CRD-myc-GPI |
|---|---|---|
| mfz3 | − | − |
| mfz4 | ++ | ++ |
| hfz5 | ++ | ++ |
| mfz6 | − | − |
| mfz7 | + | + |
| mfz8 | ++ | ++ |
| Xfz8 | ++ | ND |
| Dfz2 | ++ | ++ |
| 1. Dfz2 (66GSG67) | − | − |
| 2. Dfz2 (68GSG69) | − | − |
| 3. Dfz2 (73GSG74) | − | − |
| 4. Dfz2 (80GSG81) | − | − |
| 5. Dfz2 (85GSG86) | ++ | + |
| 6. Dfz2 (89GSG90) | − | − |
| 7. Dfz2 (92GSG93) | − | − |
| 8. Dfz2 (96GSG97) | − | − |
| 9. Dfz2 (102GSG103) | ++ | ++ |
| 10. Dfz2 (106GSG107) | ++ | ++ |
| 11. Dfz2 (111GSG112) | + | − |
| 12. Dfz2 (114GSG115) | − | − |
| 13. Dfz2 (126GSG127) | + | + |
| 14. Dfz2 (128GSG129) | ++ | ++ |
| 15. Dfz2 (130GSG131) | + | − |
| 16. Dfz2 (137GSG138) | − | − |
| 17. Dfz2 (141GSG142) | − | − |
| 18. Dfz2 (144GSG145) | ++ | − |
| 19. Dfz2 (152GSG153) | ++ | ++ |
| 20. Dfz2 (160GSG161) | ++ | + |
| 21. Dfz2 (165GSG166) | − | − |
| 22. Dfz2 (172GSG173) | ++ | + |
| 23. Dfz2 (174GSG175) | ++ | ++ |
The three amino acid insertion mutants are referred to by the codon numbers flanking the gly-ser-gly insertion. For example, 66GSG67 refers to the insertion of the nine nucleotides encoding gly-ser-gly between codons 66 and 67. ++, strong binding; +, weak binding; −, undetectable binding. ND, not determined.