Table 1.

Identification of residues of NTF2 equivalent to the active site residues of scytalone dehydratase

Scytalone dehydratase residue	Proposed function	NTF2 equivalent
Tyr-30	Assists protonation of substrates’ carbonyl	Phe-22
Asp-31	Activation of His-85	Asp-23
Tyr-50	Assists protonation of substrates’ carbonyl	Trp-41
His-85	Proton abstraction	His-66
Val-108	Involved in positioning of His-110	Leu-89
His-110	Assists in hydroxide elimination	None
Ser-129	Orientation of substrate	Phe-99
Asn-131	Orientation of substrate	Gln-101