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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Cell Signal. 2007 Nov 17;20(2):443–452. doi: 10.1016/j.cellsig.2007.11.005

Figure 3. Determination of the relative abundance of Dvl1, Dvl2, and Dvl3 in mouse F9, mouse P19, and human HEK 293 cells.

Figure 3

A, mouse F9 cells expressing Fz1 were co-transfected without or with increasing amounts of plasmid DNA (expression vector) harboring a single Dvl isoform tagged with both HA and GFP at the C-terminus of the molecule, as described. After 48 hr, the F9 cells were disrupted and sample of whole-cell lysates was subjected to SDS-PAGE and transfer of the resolved proteins to nitrocellulose blots that were then stained with primary antibodies against either Dvl1, or Dvl2, Dvl3, or the HA-antigen tag. A set of representative blots obtained from exogenously expressed Dvl isoforms (upper panels) and plots of expression of these isoforms (lower panels). B, analyses of the relative abundance of Dvl1, Dvl2, and Dvl3 in F9, P19, and HEK 293 cells. Samples (0.1 mg protein/SDS-PAGE lane) of whole-cell lysates were subjected to SDS-PAGE and transfer of the resolved proteins to nitrocellulose blots. The blots were stained with primary antibodies against Dvl1, or Dvl2, or Dvl3. A set of representative blots of endogenously expressed Dvl isoforms is displayed shown (left panels). These data were analyzed and the relative amount of Dvl isoform expressed in each cell calculated (right panel). *, p ≤ 0.01; **, p ≤ 0.001 for the difference from Dvl1 expression.