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. 2001 May 1;117(5):457–468. doi: 10.1085/jgp.117.5.457

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Activation of CFTR Cl⁻ conductance by PKA stimulation. The two-electrode voltage-clamp technique was used to measure ionic currents before and ∼30 min after exposure to a cAMP cocktail consisting of 250 μM 8-Br-cAMP and 25 μM forskolin (cAMP). (A) Representative currents obtained in water-injected oocytes and oocytes expressing hCFTR or XCFTR. (B) Time course of hCFTR and XCFTR activation by cAMP. ΔI is the current measured at 0 mV minus the current at −30 mV (holding potential). (C) Halide-selectivity sequence of cAMP-activated hCFTR. I-V relationships were obtained from an oocyte in the constant presence of cAMP cocktail by substituting NaCl in each solution with the corresponding sodium halide salt for 2 min before the I-V plots. Currents were measured at 400 ms after the start of voltage pulses ranging from −100 to 30 mV at 10-mV intervals. (D) Halide-selectivity sequence obtained from a XCFTR-expressing oocyte. Injection of hCFTR and XCFTR cRNAs elicits anion currents with the expected halide selectivity.