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. 2008 Feb 8;4(2):e22. doi: 10.1371/journal.ppat.0040022

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© 2008 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cells were co-transfected with expression vectors for indicated proteins tagged with HA (A) or FLAG (B) epitopes and the indicated protein fused with luciferase. The indicated tagged bait protein was immunoprecipated by correspondent monoclonal Ab and then the immune complex was eluted to be analysed for luciferase activity. Data presented are from one of the three independent experiments.

(C) HeLa cells were infected with vB14 and a cell extract was prepared and analysed by size exclusion chromatography on a Superose 6 column. An aliquot of each fraction was separated in 4%–12% NuPAGE and analysed by immunoblotting with the indicated Abs. The position of protein size markers is indicated in kDa at the top.

(D) Extracts from HeLa cells infected with the vΔB14 (Δ) or vB14-HA (HA) were pre-cleared and immunoprecipitated with mAbs against HA, NEMO, and GSK3β (isotype control). The immunoprecipitated proteins were resolved in 4%–12% NuPAGE and then were analysed by immunoblotting with the Abs indicated on the right, respectively. Sizes of bands detected are indicated on the left in kDa.