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HEK 293T cells were transfected with vectors expressing B14 (3, 1.2, and 0.6 μg) and HA-IKKβ (2 μg). The empty vector pCI was used to adjust the total DNA added to 5 μg per reaction per 6 cm dish. After 24 h, cell lysates were resolved by SDS-PAGE (15% gel) and analysed by immunoblotting using the Abs indicated on the right (the anti-HA mAb was used to detect total HA-IKKβ). Sizes of bands detected are indicated on the left in kDa.
