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. 2008 Feb 8;4(2):e27. doi: 10.1371/journal.pgen.0040027

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© 2008 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Fluorescent microscopy analysis of Myc-AGO4 and Cajal body localization in nuclei isolated from Myc-AGO4 seedlings. A monoclonal antibody to endogenous U2B′′ was used to detect the Cajal body. Three different AGO4 localization patterns relative to U2B′′ are shown. Nuclei containing observable AGO4 nuclear foci were examined.

(B) Localization of Myc-AGO4 and NRPD1b in Myc-AGO4 nuclei. A polyclonal antibody was used to detect endogenous NRPD1b. Three representative nuclei are shown. Nuclei containing observable AGO4 nuclear foci were examined.

(C) Localization of U2B′′ relative to NRPD1b within wild type Ler nuclei.

(D) Three color fluorescent microscopy analysis of Myc-AGO4, U2B′′-GFP, and NRPD1b localization within the same nucleus. One representative nucleus containing two different AGO4 bodies is shown: colocalization with NRPD1b (top of the nucleus) versus colocalization with the Cajal body (bottom of the nucleus). All AGO4 foci colocalized with either NRPD1b or Cajal body (n = 156).