Figure 4. X-chromosome Inactivation in E9.5 PGCs Is Still Random.
The hindgut of XX E9.5 XGFP embryos, which inherited X-linked GFP either from the mother or the father, were immunostained whole mount for Oct4 to identify the PGCs.
(A–C) White asterisks depict PGCs that do or do not co-express GFP. Note that the lumen of the hindgut shows aspecific Oct4 immunostaining.
(D) The expression of GFP was analysed in the total number of PGCs per embryo. Green bars depict the median, n is the total number of embryos analysed. Red bar depicts the median, n is the total number of embryos analysed.