Figure 2.

Modification of R334C CFTR by MTSET⁺ was stable for at least 5 h. The conductance (g_Cl@E_rev) was first obtained 30–40 min after the initial exposure of the oocytes to stimulatory cocktail when the activation of the Cl⁻ conductance had attained a steady state (control). Each oocyte was exposed to 100 μM or 1 mM MTSET⁺ for 20 s. The MTSET⁺-containing stimulatory cocktail was then replaced with stimulatory cocktail lacking the thiol reagent, and the oocytes were continuously perfused for up to 5 h. The conductances measured at 2 min, 2 h, and 5 h after exposure of oocytes to MTSET⁺ were normalized to the steady-state conductance before exposure to MTSET⁺.