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. 2001 Oct 1;118(4):433–446. doi: 10.1085/jgp.118.4.433

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The addition of new channels can be monitored by labeling preexisting channels with MTSET⁺. (A) A record of the conductance measured at the reversal potential throughout an experiment on day 1. The conductance was increased after 4.5 h due to the addition of new, unlabeled channels. (B) A record of the conductance measured at the reversal potential throughout an experiment on day 5. No increase in the conductance was observed during the 4.5-h period.