Figure 3.

Rapid ligand-dependent modulation of allosteric ribozymes. Tripartite ribozyme constructs carrying either a class I induction element (A) or a class II inhibition element (B) are depicted. Sequences for the aptamer and ribozyme domains are as shown in Fig. 1. The performance of these ribozymes in the presence and absence of FMN are evident from plots C and D, which show the natural logarithm of the fraction of ribozyme remaining uncleaved versus time relative to FMN addition. (Insets) Plots provide an expanded view of ribozyme responses to FMN addition.