Figure 1.

Microarray analysis of PPAR$\alpha$-dependent gene regulation in mouse liver. (a) Venn diagram showing the number of differentially expressed probesets between livers of 24-hour fasted wild-type and PPAR$\alpha$-null mice, and between wild-type and PPAR$\alpha$-null mice treated with the PPAR$\alpha$ agonist Wy14643 for 5 days. Pooled RNA was hybridized to Affymetrix MOE430A arrays. A fold-change of $>1.5$ was used as cutoff. (b) Venn diagram showing the number of differentially expressed probesets between livers of wild-type and PPAR$\alpha$-null mice treated with the PPAR$\alpha$ agonist Wy14643 for 6 hours, and between wild-type and PPAR$\alpha$-null mice treated with the PPAR$\alpha$ agonist Wy14643 for 5 days. RNA from individual mice was hybridized to mouse 430 2.0 arrays. Probesets that satisfied the criteria of fold-change $>1.5$ and FDR $<0.01$ were considered to be significantly regulated.