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. 2001 Jul 1;118(1):63–78. doi: 10.1085/jgp.118.1.63

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effects of different PDE inhibitors on forskolin-induced Ca²+ influx in HEK-293 cells. C460W/E583M channels were used to monitor cAMP accumulation in the presence and absence of PDE inhibitors. PDE inhibitors were added at time zero, and 1 μM forskolin was added at 180 s (indicated by arrows). The IC₅₀ for each PDE inhibitor is given in Table . Only the nonspecific PDE inhibitor IBMX (A and B) and the PDE type-IV–specific inhibitor RO-20-1724 (G) influenced the time course of forskolin-induced Ca²+ influx. In general, there was little variability in responses within a single batch of cells.