Table 3.
Effect of PDE Inhibitors on Basal cAMP Levels in GH4C1 Cells
| Inhibitor | d(ΔF/F0)/dt | No. of experiments |
|---|---|---|
| μM | s−1 | |
| 100 IBMX | 0.0025 ± 0.0009 | 5 |
| 100 8-methoxymethyl-IBMX | 0. 0029 ± 0.0011 | 4 |
| 10 EHNA | 0 | 3 |
| 1 Trequinsin | 0 | 3 |
| 15 Quazinone | 0 | 3 |
| 100 RO-20-1724 | 0 | 4 |
| 100 Rolipram | 0 | 4 |
| 50 Zaprinast | 0 | 3 |
cAMP concentration was monitored using Ca2+ influx through C460W/E583M channels. The experimental protocol is described in the legend of Fig. 8 C. d(ΔF/F0)/dt, which is proportional to the Ca2+ influx rate, was estimated as described in materials and methods. Data are given as mean ± standard error.