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. 2000 Apr 1;115(4):519–532. doi: 10.1085/jgp.115.4.519

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Enhancement of a test tetanus-induced rise in [Ca²⁺]_i after the conditioning tetanus and the blockade by ryanodine. (A) Enhancement of a test tetanus-induced rise in [Ca²⁺]_i (50 Hz, 20 pulses) after the conditioning tetanus (10 Hz, 6 min) and its blockade by ryanodine (10 μM) in a low Ca²⁺, high Mg²⁺ solution. Ryanodine was applied for 30 min. (B) Enhancement of a test tetanus-induced rise in [Ca²⁺]_i (50 Hz, 10 pulses) after the conditioning tetanus (10 Hz, 3 min) in normal Ringer solution. Test tetanus-induced rises in [Ca²⁺]_i were recorded by measuring changes in dOGB-1 fluorescence with an intensified CCD camera.