Table I.
Amino Acids Observed at Position 3 of Canonical EF-Loops
| Amino acid | Full EF-hand database* | Classical EF-hand motif‡ | Kretsinger consensus§ | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Asp | 596 | 55.5% | 482 | 73.3% | 417 | 80.3% | ||||||
| Asn | 182 | 17.0% | 161 | 24.5% | 102 | 19.7% | ||||||
| Glu | 96 | 8.9% | 2 | 0.3% | ||||||||
| Gly | 45 | 4.2% | 2 | 0.3% | ||||||||
| Thr | 30 | 2.8% | 3 | 0.5% | ||||||||
| Ser | 21 | 2.0% | 6 | 0.9% | ||||||||
| Met | 19 | 1.8% | ||||||||||
| Cys | 16 | 1.5% | ||||||||||
| Ala | 14 | 1.3% | ||||||||||
| Leu | 11 | 1.0% | ||||||||||
| Lys | 10 | 0.9% | 1 | 0.1% | ||||||||
| Gln | 9 | 0.8% | ||||||||||
| Ile | 7 | 0.6% | ||||||||||
| Arg | 6 | 0.6% | 1 | 0.1% | ||||||||
| Pro | 5 | 0.5% | ||||||||||
| Val | 5 | 0.5% | ||||||||||
| Tyr | 1 | 0.1% | ||||||||||
| Phe | ||||||||||||
| His | ||||||||||||
| Trp | ||||||||||||
| Total | 1,073 | 658 | 519 | |||||||||
*
Database courtesy of Dr. R.H. Kretsinger (Kawasaki and Kretsinger, 1994).
‡
Defined by the previously described consensus algorithim (Prosite algorithm; Falke et al., 1994) except that position 3 is allowed to vary freely.
§
Defined by the Kretsinger consensus algorithm for functional EF-hands (Linse and Forsen, 1995).