Table 3.
Upland cotton BAC and BIBAC libraries that have been published or are accessible to the public (as of May 2007).
| Genotype | Mean insert size (kb) | No. of clones | Genome equivalents | Vector(a) | Cloning site | References/locations where libraries are available |
|---|---|---|---|---|---|---|
| Tamcot HQ95 | 93 | 51,353 | 2.3x | pBeloBAC11 | HindIII | http://hbz7.tamu.edu |
| Auburn 623 | 140 | 44,160 | 2.7x | pBeloBAC11 | BamHI | http://hbz7.tamu.edu |
| Texas Marker-1 | 130 | 76,800 | 4.4x | pCLD04541 | BamHI | http://hbz7.tamu.edu |
| Texas Marker-1 | 175 | 76,800 | 6.0x | pECBAC1 | EcoRI | http://hbz7.tamu.edu |
| Maxxa | 137 | 129,024 | 8.3x | pCUGI-1 | HindIII | [79] |
| 0-613-2R | 130 | 97,825 | 5.7x | pIndigoBAC-5 | HindIII | [80] |
(a)The vectors, pBeloBAC11 (Kim et al. [82]), pECBAC1 (Frijters et al. [82]), pCUGI-1 [79], and pIndigoBAC-5 (http://www.epibio.com/item.asp?ID=328), are BAC vectors whereas pCLD04541 is plant-transformation-competent BIBAC vector (http://www.jic.bbsrc.ac.uk/staff/ian-bancroft/vectorspage.htm; [83]) that can be directly transformed into cotton plants via Agrobacterium.