Skip to main content
. 2002 May;119(5):467–486. doi: 10.1085/jgp.20028520

Figure 2.

Figure 2.

Relationship between gating charge recovery and channel closure in the wild-type and the W434F channel. The percentage of gating charge recovery was determined using the double pulse protocol by calculating the ratio of the ON gating charge in the second pulse to that in the first pulse (left). Tail currents from the left panels are shown on the middle panels on a different time scale. Solutions used in the experiments were (A) symmetrical 1 K+ + 115 Tris+ for wild-type and (B) symmetrical 115 Na+ for W434F channels. (A) In the wild-type channel the relationship between charge recovery and channel closing is nonlinear in 1 K+ solutions, (B) but it is linear in the W434F mutant in 115 Na+ solutions. In the wild-type, 13% of the charge recovered (A, left) while the tail current dropped 48% compared with its peak value (A, middle) in this patch; however, (B) in W434F a tail current loss of 54% was accompanied by 58% charge recovery. These results from many patches (WT: n = 29; W434F: n = 18) and various interpulse durations are summarized in the point plots (right).