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. 2001 Aug 1;118(2):171–182. doi: 10.1085/jgp.118.2.171

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© 2001 The Rockefeller University Press

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The effect of external Cd²+ and TTX on the cysteine substitution mutants on the NH₂-terminal side of putative selectivity filter in μ1. (A) Bar plot of the IC₅₀s for Cd²+ block of each of the mutants that express current. The mutants R395C, R750C, L746C, F745C, Y1227C, L1228C, and I1519C have IC₅₀s (mean ± SD) 999 ± 64, 201 ± 10, 813 ± 10, 123 ± 19, 650 ± 106, 292 ± 94, and 173 ± 19 μM (P < 0.05 versus WT, 1,955 ± 207 μM), respectively. (B) Bar plot of the IC₅₀s for TTX of each of the mutants that express current. The mutants R395C, F745C, R750C, and L1228C have IC₅₀s for TTX block of (mean ± SD) 112 ± 32, 1,660 ± 425, 4,431 ± 661, and 441 ± 268 nM (P < 0.05 versus WT, 13 ± 4 nM), respectively. In cases without standard error bars, full dose–response curves were obtained in individual cells, and the lack of deviation from wild-type was confirmed in several other experiments using single doses of Cd²+ (0.5 mM) and TTX (50 nM).