Table 1.

Conductance Values and Kinetic Parameters for the Interaction of βCD with WT-αHL and Met-113 Mutants

		−40 mV					+40 mV
αHL pore	Side chain	gP	gPC	kon	koff	K d	gP	gPC	kon	koff	K d
		pS	pS	M−1s−1	s−1	M	pS	pS	M−1s−1	s−1	M
WT-αHL	N	651 ± 4	240 ± 3	4.0 ± 0.3 × 105	1.3 ± 0.1 × 103	3.4 ± 0.4 × 10−3	721 ± 6	253 ± 4	2.8 ± 0.2 × 105	2.1 ± 0.2 × 103	7.8 ± 0.3 × 10−3
RL-2	N	691 ± 4	255 ± 6	2.9 ± 0.1 × 105	9.8 ± 0.3 × 102	3.3 ± 0.2 × 10−3	739 ± 7	220 ± 7	2.4 ± 0.3 × 105	1.5 ± 0.2 × 103	6.9 ± 0.1 × 10−3
M113K	+	558 ± 10	241 ± 3	3.3 ± 0.3 × 105	7.0 ± 0.5 × 102	2.1 ± 0.1 × 10−3	629 ± 8	181 ± 5	1.6 ± 0.2 × 105	2.0 ± 0.1 × 103	1.4 ± 0.1 × 10−2
M113R	+	585 ± 6	289 ± 4	4.3 ± 0.1 × 105	6.6 ± 0.2 × 102	1.6 ± 0.1 × 10−3	666 ± 8	266 ± 3	2.2 ± 0.2 × 105	3.1 ± 0.3 × 103	1.3 ± 0.1 × 10−2
M113H	+	596 ± 7	193 ± 2	3.0 ± 0.6 × 105	2.1 ± 0.4 × 10−1	7.0 ± 1.7 × 10−7	677 ± 4	189 ± 5	3.3 ± 0.4 × 105	1.1 ± 0.3 × 10−1	3.0 ± 1.0 × 10−7
M113E	−	743 ± 6	217 ± 3	1.1 ± 0.2 × 105	1.4 ± 0.1 × 103	1.2 ± 0.2 × 10−2	628 ± 7	153 ± 5	4.0 ± 0.3 × 105	1.4 ± 0.1 × 103	3.3 ± 0.5 × 10−3
M113D	−	717 ± 3	228 ± 6	2.9 ± 0.4 × 105	4.9 ± 1.0 × 10−2	1.7 ± 0.8 × 10−7	662 ± 3	211 ± 6	3.7 ± 0.7 × 105	4.1 ± 1.0 × 10−2	1.2 ± 0.4 × 10−7
M113G	P	689 ± 5	252 ± 5	2.3 ± 0.1 × 105	8.9 ± 0.8 × 102	3.7 ± 0.3 × 10−3	736 ± 9	214 ± 7	2.3 ± 0.1 × 105	2.0 ± 0.2 × 103	8.4 ± 1.7 × 10−3
M113N	P	632 ± 7	311 ± 3	2.7 ± 0.6 × 105	3.8 ± 1.1 × 10−2	1.3 ± 0.6 × 10−7	646 ± 7	297 ± 4	2.9 ± 0.6 × 105	5.0 ± 0.8 × 10−2	1.8 ± 0.8 × 10−7
M113Q	P	714 ± 11	242 ± 6	3.2 ± 0.2 × 105	1.2 ± 0.1 × 103	4.2 ± 0.2 × 10−3	769 ± 10	218 ± 3	3.2 ± 0.2 × 105	1.6 ± 0.2 × 103	5.1 ± 0.8 × 10−3
M113C	P	576 ± 7	231 ± 5	2.6 ± 0.2 × 105	1.2 ± 0.1 × 103	4.3 ± 0.2 × 10−3	651 ± 3	199 ± 6	2.0 ± 0.2 × 105	2.7 ± 0.1 × 103	1.3 ± 0.2 × 10−2
M113S	P	744 ± 6	264 ± 3	2.5 ± 0.2 × 105	8.0 ± 0.7 × 102	3.4 ± 0.4 × 10−3	761 ± 9	242 ± 4	1.8 ± 0.2 × 105	1.5 ± 0.1 × 103	8.1 ± 0.6 × 10−3
M113T	P	705 ± 9	241 ± 4	1.8 ± 0.2 × 105	8.5 ± 0.9 × 102	5.0 ± 0.6 × 10−3	761 ± 5	195 ± 3	2.3 ± 0.2 × 105	7.5 ± 0.5 × 102	3.0 ± 0.5 × 10−3
M113Y	P	682 ± 6	209 ± 5	1.8 ± 0.5 × 105	3.2 ± 0.9 × 10−2	1.9 ± 0.9 × 10−7	698 ± 12	235 ± 8	2.0 ± 0.4 × 105	3.5 ± 0.6 × 10−2	1.7 ± 0.8 × 10−7
M113A	N	658 ± 8	243 ± 5	2.3 ± 0.2 × 105	7.7 ± 0.4 × 102	3.5 ± 0.3 × 10−3	721 ± 9	209 ± 5	2.3 ± 0.2 × 105	1.5 ± 0.1 × 103	6.0 ± 0.6 × 10−3
M113V	N	722 ± 7	183 ± 7	2.3 ± 0.3 × 105	6.3 ± 0.7 × 10−1	2.8 ± 0.3 × 10−6	786 ± 7	202 ± 7	2.4 ± 0.2 × 105	3.0 ± 0.2 × 10−1	1.2 ± 0.3 × 10−6
M113L	N	666 ± 7	243 ± 3	2.7 ± 0.2 × 105	9.5 ± 0.7 × 102	3.8 ± 0.2 × 10−3	763 ± 4	237 ± 5	2.2 ± 0.2 × 105	1.6 ± 0.2 × 103	7.3 ± 0.9 × 10−3
M113I	N	637 ± 5	232 ± 5	1.4 ± 0.1 × 105	3.0 ± 0.1 × 102	2.1 ± 0.1 × 10−3	773 ± 6	273 ± 4	1.4 ± 0.1 × 105	2.6 ± 0.3 × 102	1.7 ± 0.4 × 10−3
M113P	N	816 ± 10	285 ± 4	2.1 ± 0.2 × 105	1.7 ± 0.1 × 102	8.4 ± 0.5 × 10−4	849 ± 8	246 ± 7	3.5 ± 0.2 × 105	1.1 ± 0.1 × 102	2.9 ± 0.3 × 10−4
M113F	N	624 ± 4	165 ± 2	3.1 ± 0.2 × 105	4.0 ± 0.7 × 10−2	1.2 ± 0.2 × 10−7	702 ± 5	204 ± 5	1.7 ± 0.3 × 105	8.6 ± 1.2 × 10−2	5.4 ± 0.2 × 10−7
M113W	N	524 ± 5	165 ± 5	4.2 ± 0.4 × 105	1.5 ± 0.1 × 101	3.6 ± 0.3 × 10−5	630 ± 7	192 ± 3	2.1 ± 0.3 × 105	2.0 ± 0.1 × 101	9.7 ± 2.1 × 10−5