Table 2.

Charge Selectivity of Pores and Voltage Dependence of Affinity for βCD

	Charge selectivity			Voltage dependence of affinity for βCD
αHL pore	Vr	PK+/PCl−	α	(1/K d+40)/(1/K d−40)	β
	mV
WT-αHL (pH 5.0)	−15.8	0.34	−0.45	0.25	−0.60
M113R	−13.7	0.39	−0.41	0.11	−0.96
M113K	−11.6	0.46	−0.34	0.16	−0.80
E111N	−7.2	0.63	−0.20	0.13	−0.89
WT-αHL (pH 7.5)	−3.7	0.79	−0.10	0.53	−0.28
M113T	+0.4	1.03	0.01	1.5	0.18
M113V	+1.7	1.12	0.05	2.2	0.34
E111N/K147N	+2.7	1.20	0.08	1.3	0.10
M113P	+3.3	1.24	0.09	2.6	0.42
K147N	+11.5	2.19	0.34	2.9	0.46
WT-αHL (pH 11.0)	+11.9	2.20	0.34	6.6	0.82
M113D	+12.4	2.34	0.37	1.5	0.18
M113E	+13.5	2.54	0.40	3.6	0.56