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. 2001 Nov 1;118(5):563–582. doi: 10.1085/jgp.118.5.563

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Western blots of neuroblastoma AChRs compared with AChRs expressed in permanently transfected HEK cells. AChRs in Triton X-100 extracts were immunoisolated on mAb-coated resin. α3-containing AChRs were isolated by mAb 210. β2-containing AChRs were isolated by mAb 295. β4-containing AChRs were isolated by mAb 337. Proteins were separated by electrophoresis in SDS on a 12% polyacrylamide gel. α3 subunit protein was bound with rat antisera raised against the α3 subunit and then labeled with ¹²⁵I-GART. β4 subunit protein was labeled directly with ¹²⁵I-mAb 337.