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. 2001 Nov 1;118(5):563–582. doi: 10.1085/jgp.118.5.563

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The single-channel properties for α3β4 AChRs from transfected HEK cells are nearly identical to those for α3β4 AChRs expressed in Xenopus oocytes and for IMR-32. Representative recordings of the single-channel currents for α3β4 AChRs expressed in permanently transfected HEK cells are shown along with representative event amplitude and dwell time distributions. Superimposed on the event histograms are fits to either a double Gaussian function for the amplitude distribution or a double exponential function for the open time distribution. The recordings were made in the outside-out patch configuration using ACh (5 μM) as agonist at -80 mV. Mean channel open times were determined as described in materials and methods. The single-channel amplitudes and open times were nearly identical to those for oocyte-expressed AChRs and for IMR-32 AChRs recorded under the same conditions (Nelson and Lindstrom 1999; see preceding page).