Table 1.
Predicted and Observed Fractions of Cd2+-inhibited HCN Current with Mixed Tandem Dimers
| Experiment | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | |
| Transfected cDNA ratio (CC:TT) | 1:1 | 1:1 | 1:3 | 1:1 |
| Fraction irreversibly inhibited current | 0.64 | 0.60 | 0.27 | 0.71 |
| Inferred CC:TT dimer expression ratio | 4:1 | 3.3:1 | 1.1:1 | 5.3:1 |
| Predicted fraction TT/TT channels | 0.04 | 0.05 | 0.23 | 0.025 |
| Predicted fraction TT/CC channels | 0.32 | 0.35 | 0.50 | 0.265 |
| Predicted fraction reversibly inhibited current | 0.27 | 0.30 | 0.42 | 0.23 |
| Observed fraction reversibly inhibited current | 0.25 | 0.20 | 0.39 | 0.21 |