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. 2002 Jun;119(6):571–580. doi: 10.1085/jgp.20018544

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — 7D5 antibody staining of COS_phox cells compared with COS_WT cells. Cells harvested by brief trypsinization were incubated with 7D5 monoclonal antibody, which recognizes an extracellular epitope of gp91^phox. Following incubation with a FITC-conjugated goat anti–mouse secondary antibody, cells were analyzed by flow cytometry. The marker M1 was set to exclude 99% of the COS_WT cells (open histogram), and indicates the range defined as positively stained. By this criterion, 98.3% of the COS_phox cells (solid histogram) were positively stained for gp91^phox expression.