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. 2002 Jun;119(6):533–543. doi: 10.1085/jgp.20028514

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Stretch-induced Ca²⁺ release occurs through RYR gating. (A) Control experiment demonstrating a stretch-activated Ca²⁺ wave following cell stretch. Images shown are before, during, and after the second stretch of a mouse bladder myocyte. The fluorescence profile from the region of the initiation of the Ca²⁺ wave is shown for the entire experiment below. Pixel size = 0.95 μM. (B) Similar experiment in a mouse cell incubated with 2-APB (100 μM) to inhibit InsP₃R demonstrates SICR in the form of Ca²⁺ sparks, despite the complete block of Ca²⁺ release by methacholine (mACH, 50 μM), indicating sufficient InsP₃R block. (C) Incubation with ryanodine (10 μM) abolished SICR in a rabbit myocyte and this effect was not due to SR depletion, since methacholine was capable of releasing Ca²⁺.