Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Sep;124(3):273–287. doi: 10.1085/jgp.200409040

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Exogenous H₂O₂ activates Cl⁻ SAC in the absence of integrin stretch. Currents before (A, Control) and after (B, H₂O₂) a 7-min exposure to 500 μM H₂O₂, and after application of 10 μM tamoxifen for 6 min in the continued presence of H₂O₂ (C, +Tamoxifen). The H₂O₂-induced difference current (D, H₂O₂-Induced Difference) was obtained by digital subtraction. (E) I–V relationships for A–C, and for the H₂O₂-induced current (F). Each I–V relationship reversed near E_Cl. The H₂O₂-induced current partially inactivated at positive potentials and exhibited strong outward rectification. Tamoxifen, an inhibitor of Cl⁻ SAC and I_Cl,swell, blocked all of the H₂O₂-induced Cl⁻ current, as well as a large fraction of the background Cl⁻ current. At +40 mV, the H₂O₂-induced Cl⁻ current was 1.00 ± 0.15 pA/pF (n = 4), and tamoxifen blocked this current by 121 ± 15% (n = 4). Inset, chemical structure of tamoxifen.