Figure 3.

Evolution of dye and H⁺ concentrations in the working fiber segment. Time was measured from the moment of permeabilization of cut fiber ends, when fiber was first exposed to internal solution containing ApIII (0.8 mM) and phenol red (1.25 mM). [ApIII] and [phenol red] were derived from resting absorbances at 480 and 620 nm (Eq. 1). [H⁺] from measurements at 575 nm. Dashed lines mark the interval during which the depletion pulse pattern was applied. Identifier 1726.