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. 2004 Sep;124(3):239–258. doi: 10.1085/jgp.200409071

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Initial analysis of optical records. (A) Changes in transmitted light intensity. I, simultaneously measured at three wavelengths as indicated. (B) Dye-related changes in absorbance at 575 and 720 nm, derived from intensities in A. Record ΔA _Ap(720) is derived (according to Eq. 4) by linear combination of ΔA(720) and ΔA _intrinsic(720), which in turn is derived from ΔI(550), blue in A, and I(550). ΔA _PR(575) (dashed) is derived according to Eq. 6, by linear combination of A(575), A _Ap(575), and A _intrinsic(575) (in turn evaluated by Eq. 5). A _Ap(575) is calculated as the sum of a resting component (second term, right hand side of Eq. 2) and a change ΔA _Ap(575), derived by scaling ΔA _Ap(720) by 0.25. The record in red, continuous trace, is ΔA(575) after correction for the intrinsic absorbance change (i.e., the total change in absorbance due to both dyes). It is very similar to ΔA _PR(575), which stresses that the interference between the two dyes is almost negligible. Identifier 1723, vertical diameter = 73 μm, [phenol red]= 2.20 mM, [ApIII]= 0.86 mM, pH 6.35.