Figure 2.
Analytical size-exclusion chromatography of human p53tetS and multiple mutants with the hydrophobic cores of human p51 and p73. The column was calibrated by using a set of molecular weight markers as shown in the lower part of the figure. The peaks correspond to thyroglobulin (660 kDa, V0), BSA (67 kDa), ovalbumin (43 kDa), chymotrypsin (25 kDa), ribonuclease A (13.7 kDa), aprotinin (6.5 kDa), and acetone (Vt). p53tet chromatograms are shown on the upper part and have been offset for clarity. They correspond (from top to bottom) to human p53tetS and mutants L332V/M340I/F341L (core from p73), and F328L/L332V/F341L/l344I (core from p51). The initial (monomer) concentration was 40 μM.