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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Exp Parasitol. 2007 Aug 29;118(1):103–110. doi: 10.1016/j.exppara.2007.07.016

Fig. 2.

Fig. 2

Telomere size distribution among chromosome classes analyzed by 2-dimensional gel electrophoresis. (A) Chromosome size polymorphism among T. brucei clones RUMP151, 102, 100, TREU927 and Lister 427 SM. Chromosomes were separated by Rotating Agarose Gel Electrophoresis and stained with Ethidium bromide. (B) Entire lanes from (A) were digested with a mixture of MboI, AluI and RsaI, and perpendicularly embedded in 0.8% agarose to separate telomere terminal restriction fragments (Ethidium-bromide-stained chromosomal DNA profiles were graphically inserted on top of the gel). Telomere restriction fragments were visualized using a (TTAGGG)4-probe. MBC are well separated (arrows) whereas IC and MC (circled) are not. Exposure time was 6 h. (C) Direct comparison of Lister 427-SM parental and extensively propagated telomerase-deficient clone. Exposure time was 15 hours. Size markers are as indicated. It is unclear whether the genomic rearrangements (*) in the TERT null clone are derived from MC or IC.