Table 1.
Fungal cultures, rDNA sequences, QPCR assays and calibration standard curve parameters used for target organism quantification
| Standard cultures and rDNA sequences
|
QPCR assays and standard curve parameter values
|
||
|---|---|---|---|
| Species, strain, GenBank accession # | Assay name | Slope (b) | y-intercept (a) |
| Acremonium strictum, ATCC 34717, cf. AY625058 | Astrc | −3.73 | 23.83 |
| Alternaria alternata, EGS 35-193, cf. AY625056 | Aaltr | −4.00 | 23.76 |
| Aspergillus caespitosus, SRRC 308, AY373841 | Acaes | −3.53 | 19.85 |
| Aspergillus candidus, NRRL 303, cf. AY373842 | Acand3 | −3.39 | 20.23 |
| Aspergillus carbonarius, SRRC 15, AY373844 | Acarb | −3.58 | 20.40 |
| Aspergillus cervinus, SRRC 371, AY373845 | Acerv | −4.23 | 32.73 |
| Aspergillus clavatus, SRRC 17, cf. AY373845 | Aclav | −3.92 | 26.33 |
| Aspergillus flavipes, NRRL 302, cf. AY373849 | Aflvp2 | −3.85 | 26.28 |
| Aspergillus flavus, ATCC 16883, cf. AY373848 | Aflav | −3.61 | 22.74 |
| Aspergillus fumigatus, NRRL 163, cf. AY373851 | Afumi | −3.56 | 20.96 |
| Aspergillus niger, ATCC 16888, AY373852 | Anigr | −3.38 | 21.01 |
| Aspergillus niveus, SRRC 333, AY373853 | Anive | −3.90 | 28.37 |
| Aspergillus ochraceus, NRRL 398, AY373856 | Aochr1 | −3.60 | 23.44 |
| Aspergillus paradoxus, SRRC 336, AY373860 | Apard | −3.47 | 19.74 |
| Aspergillus parasiticus, NRRL 502, AY373859 | Apara | −3.50 | 24.07 |
| Aspergillus penicillioides, ATCC 16910, AY373862 | Apeni2 | −3.55 | 23.13 |
| Aspergillus puniceus, SRRC 2155, AY373862 | Apuni | −3.53 | 22.04 |
| Aspergillus restrictus, ATCC 16912, AY373864 | Arest | −3.83 | 25.35 |
| Aspergillus sclerotiorum, ATCC 16892, AY373866 | Asclr | −3.56 | 22.30 |
| Aspergillus sydowii, NRRL 250, AY373866 | Asydo3 | −3.39 | 25.29 |
| Aspergillus tamarii, NRRL 427, cf. AY373870 | Atama2 | −3.67 | 24.12 |
| Aspergillus terreus, ATCC 1012, AY373871 | Aterr2 | −3.46 | 23.06 |
| Aspergillus unguis, SRRC 344, AY373872 | Aungu | −3.29 | 21.16 |
| Aspergillus ustus, NRRL 275, AY373877 | Austs2 | −3.45 | 21.09 |
| Aspergillus versicolor, NRRL 238, cf. AY373882 | Avers2 | −3.71 | 27.24 |
| Aspergillus wentii, NRRL 377, cf. AY373884 | Awent | −4.63 | 30.76 |
| Aureobasidium pullulans, EPA 701, AY625057 | Apull | −3.38 | 20.93 |
| Chaetomium globosum, ATCC 32404, cf. AY625061 | Cglob | −3.52 | 20.28 |
| Cladosporium cladosporioides 1, ATCC 6721, AY625059 | Cclad1 | −3.42 | 20.30 |
| Cladosporium cladosporioides 2, ATCC 16022, AY625060 | Cclad2 | −3.42 | 19.75 |
| Cladosporium herbarum, ATCC 28987, AY625062 | Cherb | −3.53 | 19.00 |
| Cladosporium sphaerospermum, UAMH 7686, AY625063 | Cspha | −3.57 | 20.30 |
| Emericella nidulans, NRRL 2395, AY373888 | Anidu2 | −3.69 | 24.32 |
| Emericella variecolor, SRRC 268, AY373893 | Avari | −3.92 | 24.65 |
| Eurotium amstelodami, NRRL 90, AY373885 | Eamst | −3.46 | 20.56 |
| Epicoccum nigrum, UAMH 3247, AY625063 | Enigr | −3.49 | 21.99 |
| Memnoniella echinata, UAMH 6594, AF081470 | Mem | −3.69 | 20.77 |
| Mucor racemosus, NRRL 1428, AY625074 | Muc1 | −3.32 | 18.82 |
| Paecilomyces variotii, ATCC 22319, AY373941 | Pvari2 | −3.45 | 20.97 |
| Penicillium atramentosum, NRRL 795, AF033492 | Patra | −3.77 | 25.82 |
| Penicillium aurantiogriseum, FRR 971, AY380455 | PenGrp1 | −4.04 | 25.33 |
| Penicillium brevicompactum, FRR 862, AY373898 | Pbrev | −3.97 | 25.91 |
| Penicillium canescens, FRR 910, AY373901 | Pcane2 | −3.81 | 27.02 |
| Penicillium chrysogenum, EPA 467, AY373903 | Pchry | −3.50 | 22.43 |
| Penicillium citreonigrum, FRR 2046, AY373908 | Pcteo | −3.51 | 29.15 |
| Penicillium citrinum, FRR 1841, AY373904 | Pcitr | −4.22 | 27.49 |
| Penicillium coprophilum, NRRL 13627, AF033469 | Pcopr | −3.85 | 29.03 |
| Penicillium corylophilum, FRR 802, AY373906 | Pcory | −3.72 | 24.36 |
| Penicillium crustosum, FRR 1669, AY373907 | PenGrp2 | −4.27 | 27.46 |
| Penicillium decumbens, FRR 741, AY373909 | Pdecu2 | −4.28 | 28.26 |
| Penicillium digitatum, FRR 1313, AY373910 | Pdigi | −3.63 | 22.82 |
| Penicillium expansum, ATCC 7861, AY373912 | Pexpa | −3.47 | 25.32 |
| Penicillium fellutanum, FRR 746, AY373913 | Pfell | −3.99 | 29.49 |
| Penicillium glandicola, FRR 2036, AY373916 | Pglan | −3.59 | 24.98 |
| Penicillium griseofulvum, FRR 3571, AY373917 | Pgris | −3.45 | 23.61 |
| Penicillium implicatum, FRR 2061, AY380455 | Pimpl | −3.62 | 22.17 |
| Penicillium islandicum, NRRL 10127, cf. AY373919 | Pisla | −3.49 | 22.22 |
| Penicillium italicum, ATCC 48114, AY373920 | Pital | −3.42 | 26.66 |
| Penicillium melinii, FRR 2041, AY373923 | Pmeli | −3.48 | 19.10 |
| Penicillium miczynskii, FRR 1077, AY373924 | Pmicz | −3.59 | 24.23 |
| Penicillium olsonii, NRRL 28496, cf. AY373925 | Polsn | −3.78 | 24.63 |
| Penicillium oxalicum, NRRL 787, AF033438 | Poxal | −3.96 | 25.74 |
| Penicillium purpurogenum, FRR 1061, AY373926 | Ppurp | −3.68 | 22.85 |
| Penicillium raistrickii, FRR 1044, AY373927 | Prais3 | −3.58 | 23.42 |
| Penicillium restrictum, NRRL 1748, AF033457 | Prest2 | −3.56 | 22.93 |
| Penicillium roquefortii, FRR 849, AY373929 | Proqu | −4.24 | 26.34 |
| Penicillium sclerotiorum, FRR 2074, AY373930 | Psclr | −3.32 | 19.48 |
| Penicillium simplicissimum, NRRL 1075, AF033440 | Psimp2 | −3.42 | 23.74 |
| Penicillium spinulosum, FRR 1750, AY373933 | Pspin2 | −3.32 | 24.81 |
| Penicillium variabile, FRR 1290, AY373936 | Pvarb2 | −3.34 | 20.52 |
| Rhizopus stolonifer, ATCC 14037, AY625075 | Rstol | −3.62 | 18.51 |
| Scopulariopsis brevicaulis, UAMH 7771, AY625065 | SCbrv | −3.39 | 19.24 |
| Scopulariopsis chartarum, ATCC 16279, AY625066 | SCchr | −3.39 | 20.02 |
| Stachybotrys chartarum, UAMH 6417, AF206273 | Stac | −3.55 | 18.96 |
| Trichoderma asperellum, ATCC 38501, cf. AJ230669 | Taspr1 | −3.32 | 21.51 |
| Trichoderma atroviride, EPA 405, AY625067 | Tviri | −3.75 | 22.92 |
| Trichoderma harzianum, NRRL 13019, AY625068 | Tharz | −3.89 | 25.70 |
| Trichoderma longibrachiatum, UAMH 9515, AY625069 | Tlong | −3.42 | 22.68 |
| Ulocladium atrum, EGS 30-188, cf. AY625072 | Uatrm | −3.61 | 17.01 |
| Ulocladium chartarum, EGS 36-055, cf. AY625071 | Uchar | −3.63 | 22.46 |
| Ulocladium botrytis, EGS 13-030, cf. AY625070 | Ubotr | −3.58 | 18.75 |
| Wallemia sebi, UAMH 7897, AY625073 | Wsebi | −3.43 | 21.34 |