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. 2004 Nov;124(5):445–461. doi: 10.1085/jgp.200409034

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — A single human Ca_V2.1 channel in the slow gating mode spends longer intervals of time in closed states and activates at more positive voltages than in the fast gating mode. Single channel recordings as in Fig. 1 on HEK293 cells stably coexpressing human Ca_V2.1α₁ (α_1A-2), α_2bδ-1, and β_1b, β_2e, or β_4a subunits. (A) Time constants (τ_open and τ_closed) and relative areas (%) of the exponential components best fitting the open and closed time distributions at +30 mV of single Ca_V2.1 channels (α_1A-2–β_1b–α_2bδ-1) in the slow (dark bar) and the fast (empty bar) gating mode. Average values were obtained from single channel patches (n = 5 for both the slow and fast mode) in which, by visual inspection, the channel showed only (or mainly) one gating mode or two clearly separated periods in the two modes. Average single channel current and conductance were identical for the five channels in either the slow or fast gating mode. Statistical significance of difference between paired values using student t test: *, P < 0.05; **, P < 0.001. (B) Normalized open probability, p_o, as a function of voltage of single Ca_V2.1 channels containing different β subunits gating in the fast (empty symbol) and the slow mode (dark symbol). Average values were obtained from single channel patches in which the channel showed only (or mainly) one gating mode or two clearly separated periods in the two modes: n = 5, 5, and 6 for the slow mode and n = 5, 4, and 4 for the fast mode of channels containing the β_1b, β_2e, and β_4a subunits, respectively.